Drug discovery
Photo-CIDNP NMR for fragment-based drug discovery: find binders, then measure how strongly they bind.
Fragment screening
A fragment that binds the target loses its photo-CIDNP signal. Each sample takes one scan of 2 to 5 s.
5 µM ligand2 µM target1,500 samples per day
Torres et al., J. Am. Chem. Soc. 2023Screening →Affinity determination
Dissociation constants in the micromolar to millimolar range, from a short titration of the target.
5 to 15 min0.1 mg protein
Bütikofer et al., J. Am. Chem. Soc. 2024Affinity determination →